human egfr-fc fusion protein  (GenScript corporation)

Journal: PLoS ONE

Article Title: Inhibition of Cell Proliferation by an Anti-EGFR Aptamer

doi: 10.1371/journal.pone.0020299

Figure Lengend Snippet: The N62 pool and aptamers E03, E04, and E07 were assayed in triplicate by filtration for binding to hEGFR, mEGFR, hErbB2, and hIgG1. Average values and standard deviations are shown. Binding assays were carried out either in the absence (left) or presence (right) of DTT. A no protein control was also carried through the procedure. Percent binding was relative to the total RNA added.

Article Snippet: About 2 nmoles RNA and 90 pmoles recombinant human EGFR-Fc (hEGFR) fusion protein (R&D Systems, Minneapolis, MN) were used for each round of selection in a reaction volume of 100 μL.

Techniques: Filtration, Binding Assay, Control

Journal: PLoS ONE

Article Title: Inhibition of Cell Proliferation by an Anti-EGFR Aptamer

doi: 10.1371/journal.pone.0020299

Figure Lengend Snippet: Binding isotherms were constructed using 0.1 nM aptamer and varying amounts of hEGFR or mEGFR. Binding assays were carried out in triplicate and the average value and standard deviation are shown. The fact that binding does not reach 100% is a function of the filtration assay, and is commonly observed. Dissociation constants were calculated following curve-fitting, as described in .

Article Snippet: About 2 nmoles RNA and 90 pmoles recombinant human EGFR-Fc (hEGFR) fusion protein (R&D Systems, Minneapolis, MN) were used for each round of selection in a reaction volume of 100 μL.

Techniques: Binding Assay, Construct, Standard Deviation, Filtration